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The resulting trimmed and de-duplicated reads were then aligned to the Rattus norvegicus Rnor6.0 reference genome, available on ENSEMBL, using STAR aligner v.2.5.2b4 25 , producing BAM files

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Once the 5mg bottle of BPC-157 is full, then based on this very handy Peptide Mixing & Dosing Calculator, each time you inject a 1ml/1cc syringe into it and pull that syringe back to the eight tick mark (15 Unit mark), you are going to have yourself approximately 250mcg of BPC-157

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doi:10.1016/j.jconrel.2023.01.015