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We did not observe metabolite-induced aggregate formation in subthreshold PolyQ 19 or GFP only strains (data not shown), indicating that the metabolite alters the ability of threshold-state animals to handle polyQ misfolding

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2.15.2 Neural function defect score (NSS) Twenty-four hours post-infarction, neurological deficits were scored using the Masao Shimizu Sasamata method ( 2.16 Immunohistochemical analysis Mice were humanely sacrificed by deep anesthesia using an overdose of pentobarbital, followed by transcardial perfusion with phosphate-buffered saline (PBS) to clear the blood

Dickey, S