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For the extraction, methanol and internal standards were added, followed by incubation for 15 min at 30C on a thermal shaker (650 rpm, TS1 ThermoShaker, Biometra, Gttingen, Germany), centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany), evaporation, and centrifugation with methanol again

If this doesnt give a clear result, other tests, like an array comparative genomic hybridization (aCGH), might be needed

Metabolism alteration in follicular niche: the nexus among intermediary metabolism, mitochondrial function, and classic polycystic ovary syndrome

Quintas-Cardama A, Kantarjian HM, Ravandi F, Foudray C, Pemmaraju N , Kadia TM, Borthakur G, Daver NG, Faderl S, Jabbour E, Cortes JE, Garcia-Manero G