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Anti-FLAG M2 magnetic beads were pre-coupled to T6B-3xFLAG peptide, washed to remove unbound peptide and then incubated with clarified lysate from MEFs or S2 cells that were lysed in 50 mM Tris pH 8.0, 150 mM NaCl, 5 mM EDTA, 10% (v/v) glycerol and 0.5% (v/v) NP-40, with 1 tablet of cOmplete Mini EDTA-free Protease Inhibitor Cocktail per 10 ml of buffer

Behavioral measures: The following standardized behavioral measures were completed by primary caregivers for both the ASD and TDC groups: the Adaptive Behavior Assessment System-II (ABAS-II)( Harrison: Adaptive Behavior Assessment System - Google Scholar , n.d.) or the updated version, the ABAS-3 (N=34), and The Social Responsiveness Scale (SRS-2)( Constantino: Social Responsiveness Scale: SRS-2 - Google Scholar , n.d.) groups

10.1186/s13046-018-0843-y 171 MooreL

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221, e20221839 (2024)