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( 2 ) When primers flank a long (e.g., 1 kb) intron, the amplification cannot occur because the short extension time is sufficient for the short cDNA sequence but not for the longer genomic target

it's mechanical

[DOI] [PMC free article] [PubMed] [Google Scholar] 275.Gao X., Talalay P
A sterile aqueous solution of vitamin B12. Commonly used as supportive treatment and appetite stimulant for sick or convalescing animals
