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For each analysis, the statistical test used and n for each group are found in Supplemental Tables 2, 3, or 5

By blocking NNMT, 5-amino-1MQ restores optimal NAD+ and SAM levels, improving cellular function before any external changes become visible

This reaction simultaneously depletes two critically important metabolic cofactors: SAM (the universal methyl donor for hundreds of epigenetic and biosynthetic reactions) and nicotinamide (the precursor for NAD+ biosynthesis via the salvage pathway)

However, this remains a preclinical finding that has not been confirmed in human pharmacokinetic studies

Its core physiological function is to catalyze the methylation of nicotinamide (NAM, a key precursor for NAD+ synthesis) with Sadenosylmethionine (SAM) to produce 1methylnicotinamide (1MNA)