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Keep in a dry and cool place away from direct sunlight, heat and moisture, at a temperature below 25C
Method Enzymol 268:331340 [Google Scholar] Palmer RM, Ferrige AG, Moncada S (1987) Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor
For neutrolization step, the slides were kept in 100 mM Tris-HCl for 5 min
Moreover, 2 participants in the LC group denied continuing the trial
