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Male SAMP8 mice develop LV hypertrophy with diastolic dysfunction as early as 6 months of age ( 2.2.3 Fischer 344 rats (H 2 FPEF score 3) The aged Fischer 344 rats develop pathophysiology of aging in various organs, including the heart (Walker et al., 2006) with evident pulmonary congestion and exercise intolerance at 25 months of age (Suen et al., 2019) by 28 months of age, these animals also develop LV systolic dysfunction and chamber dilation, which is infrequently seen in human HFpEF (Walker et al., 2006), thus limiting the use of Fischer 344 rats as an HFpEF model

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The collected slides were stained with Hematoxylin and Eosin (H&E) for histological inspection using a LEICA (DFC290 HD system digital camera, Heerbrugg, Switzerland) attached to a light microscope with 10, 20, and 40 objective lenses 49

Among the nutraceuticals most investigated as single agents, yohimbine has been used for over several decades as a therapeutic option for ED [21], being particularly successful in patients with non-organic forms [22], whereas Korean red ginseng has been suggested to improve ED, although with less firm evidence [23]