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[6] [7] [8] Complex I deficiency with recessive spastic paraparesis has also been linked to MTHFR variants

Reactions were performed with 50 M RHKKAc, a fluorogenic peptide substrate from p53 residues 379-382, 500 M NAD+ co-substrate, and recombinant human SIRT-1 (NAD+-dependent) enzyme

For others, meaningful change takes the full 4-6 week loading cycle