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Single-cell lineage tracing of metastatic cancer reveals selection of hybrid EMT states

VAMP4, Vesicle Associated Membrane Protein 4

This approach is explored with small molecule drug erastin (named after eradicator of RAS and ST-expressing cells) identified to efficiently kill human tumor cells without affecting their isogenic normal cell counterparts [160]

The number of viable cells was assessed by measuring the absorbance at 450 nm using a Synergy 2 Multi-Mode Reader (Bio Tek Instruments, Inc., Winooski, VT, USA)

Cells were imaged using an InCell 2500 Analyser high-throughput microscope (GE Healthcare), as previously described