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Blots were detected using an enhanced chemiluminescence reagent [22]

Int J Clin Lab Res 29:3640 Hug G, McGraw CA, Bates SR, Landrigan EA (1991) Reduction of serum carnitine concentrations during anticonvulsant therapy with phenobarbital, valproic acid, phenytoin, and carbamazepine in children

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Native IGF-1 has a frustrating pharmacokinetic limitation: it binds tightly to a family of IGF binding proteins (IGFBPs, particularly IGFBP-3) that sequester it in circulation and keep its free half-life to roughly 15-20 minutes