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Then, we added 1% TBA and boiled the samples (95 C, 60 min)
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In brief, HUVECs were lysed in Co-IP buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, and 0.5% protease inhibitor cocktail on ice for 20 min
It was concluded, that astrocytes are responsible for the recruitment of microglia
Open 11 , e5279 (2023)
