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At t 0 (after the rehydration process), total RNA was extracted from each animal using the MasterPure Kit (Epicentre), and then used to synthesise cDNA using the SuperScript III First-Strand Synthesis System for RT-PCR (Thermo Fisher), following the manufacturers protocol

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CrossRef Gallstones and the Risk of Gallbladder Cancer Mortality: A Cohort Study Seungho Ryu, Yoosoo Chang, Kyung Eun Yun, Hyun-Suk Jung, Jun Ho Shin, Hocheol Shin American Journal of Gastroenterology.2016

Outline Part I Evolution of the Adipokine Hypothesis for Understanding HFpEF 1270 Part II Overlap of the Features of Obesity and HFpEF Point to a Common Mechanism 1275 Part III Parallelism of Nutrient Sensing Pathways in Adipose Tissue and the Heart 1278 Part IV Key Lines of Evidence Supporting the Adipokine Hypothesis of HFpEF 1279 Part V Domain I Adipokines: Suppressed Cardioprotective Molecules 1282 Part VI Domain II Adipokines: Compensatory Cardioprotective Molecules 1286 Part VII Domain III Adipokines: Prohypertrophic and Proinflammatory Molecules 1296 Part VIII Testing the Adipokine Hypothesis of HFpEF 1313 Part IX The Adipokine Hypothesis of HFpEF: Synthesis of the Evidence 1321 For the past 3 decades, the neurohormonal hypothesis has represented a unifying framework to explain heart failure with a reduced ejection fraction (HFrEF)

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