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After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min

The chemical inducers of ARE-driven gene expression modify reactive cysteine residues in Keap1

Isotretinoin and serum lipids: studies on fatty acid, apolipoprotein and intermediary metabolism

Yamamoto H, Sohmiya M, Oka N, Kato Y

The terminal alkyne containing compouds was subsequently conjugated to a thermally sensitive oxanorbornadiene linkage and supermagnetic nanostructure particle (SPION) (Figure 117A)