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Avan A, Postma TJ, Ceresa C, et al

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The SPTlike motif appears to reside close to where the regulatory and kinase/catalytic domains are and deletions in the regulatory or kinase domains of PKC are shown to affect nuclear translocation.95, 96 Mutation studies substituting the SPT motif in PKC with an alternative motif that cannot be phosphorylated impaired the localization of PKC to the nucleus, suggesting that phosphorylation of SPT is required for nuclear translocation.94 Interestingly, mutating the putative NLS motif has no effect on the distribution of PKC in the cell, suggesting that the SPTlike motif may be an alternative NLS motif used by signalling kinases that do not have the canonical NLS for nuclear transport.94 Hence, it would be of interest to examine how PKC activation can affect the configuration of the protein folding/structure and as a consequence the exposure of the nuclear localization motifs for regulation