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In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min

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As expected, SLC25A40, another recently identified mitochondrial GSH transporter, was the top hit in GshF- expressing SLC25A39 -knockout cells compared to those expressing mito-GshF irrespective of BSO treatment (Fig

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Furthermore, a reduction in corticosterone levels was recorded soon after exposure to DSIP