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Glutathione and Parkinson's disease: is this the elephant in the room

The concentration of GSH can be then determined by subtracting GSSG from tGSH

The G-site is predominantly contained in the N-terminal domain and binds GSH and primes the thiol sulfur for nucleophilic attack on an electrophilic substrate [77-79]

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In an alternative embodiment, the wash solution lacks calcium and may lack magnesium or may lack many if not all divalent cations
