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These measurements can be easily combined with microscopy analysis in the presence of fluorescence-labeled proteins to examine protein assembly [185], pore formation [186], and lipid dependence [187], as in the case of the single-molecule study of assembly and membrane insertion of PFO [188]
The key findings from this clinical program include significant reductions in body weight and fat mass in treatment groups compared to placebo, preferential loss of abdominal and visceral adipose tissue, no changes in serum IGF-1 levels at any dose tested, no negative effects on glucose metabolism or insulin sensitivity, no significant adverse events related to metabolic or endocrine disruption, and safety profile indistinguishable from placebo for most parameters
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