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For synthetic peptides, the general guidance is to avoid them under 18

Histological analyses were conducted under a light microscope (Leica CTR500, Leica Microsystems Inc., Milan, Italy) and images were captured using a high-resolution digital camera (Leica DC300F) at a magnification of 20X

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After 68 h, the supernatant was removed and replaced with fresh culture medium, followed by laser irradiation 46 h