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In a Phase II trial in pancreatic cancer patients, oral curcumin was well tolerated and showed some biological activity in a subset of patients, despite low systemic absorption (Dhillon et al., 2008)

Given the multitude of Arabidopsis GSTs (Dixon and Edwards 2010), we did not aim at heterologous expression and in vitro testing for single GSTs, but decided to non-selectively co-purify endogenous GSTs of the tau and phi subfamilies from Arabidopsis leaves using immobilized GSH affinity chromatography (Edwards and Dixon 2005)

EMSA EMSAs were performed by mixing increasing amounts of wt or mutated BRD4 ET (with or without ASXL1 peptide at a 1:1 molar ratio) with 10 nM/lane of 147 bp 601 DNA in 2025 mM Tris pH 7.5, 150 mM NaCl and 5 mM DTT in a 10 L reaction volume

doi: 10.1016/S0024-3205(03)00291-1

Statistical analysis was performed using unpaired Students two-tailed t -test using GraphPad Prism v.6 software