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Chymase-mediated intestinal epithelial permeability is regulated by a protease-activating receptor/matrix metalloproteinase-2-dependent mechanism

[PMC free article] [PubMed] [Google Scholar] 54.Pan JW, Williamson A, Cavus I, Hetherington HP, Zaveri H, Petroff OAC, Spencer DD

Criado-Gonzalez M, Iqbal MH, Carvalho A, Schmutz M, Jierry L, Schaaf P

RNA interference We transfected shRNA into extracted epithethial cells using Lipofectamine 3000 (#L3000008, Thermo Fisher, USA) according to the manufacturers instruction

Our approach combines the physicochemical properties of amino acids in the peptide which favour T-cell receptor (TCR) binding along with factors important for antigen presentation and processing (expression of the mutant gene in the tumour, HLA type, HLA binding affinity and proteasomal cleavage and processing of the peptide)
