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The shRNA sequence (TRCN0000322995) targeting ATG10 was designed according to MISSION shRNA Plasmid DNA (MERCK) and synthesized and joined the FastDigest BshTI/FastDigest EcoRI linearized (Thermo Fisher) pLKO.1-GST DAF vector with T4 DNA ligase (EL0011, Thermo Fisher)
10.1016/j.neuron.2012.08.022 Neuron 38 MengD.YangQ.MelickC
In fact, research has shown that oocytes with higher levels of intracellular glutathione produce healthier, stronger embryos
