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Patients with OA exhibit elevated iron levels in synovial tissue, and the impairment of iron homeostasis-regulating genes causes cellular iron accumulation, resulting in heightened production of ROS as well as the increased level of collagen II and MMP13, which are established hallmarks of OA [85] (Figure 2)

However, the most effective approach is not choosing one over the other, but understanding how they work together

iNOS western blotting To assess iNOS expression, cells were collected 24 h after treatment with LPS (500 ng/ml) alone or in combination with equal amount of GSSEA, BeS (10 M), or NPBeS (10 M) and lysed in a 50 mM Tris-HCl buffer containing 150 mM NaCl, 0.05% SDS, 1% Triton X-100, and a protease inhibitor cocktail (Thermo Fisher Scientific)

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Stress Management Chronic stress disrupts hormonal balance and can accelerate hair shedding