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Homogenate was divided among 6 ultracentrifuge tubes, wherein 13 mL of homogenate was gently layered onto 17 mL of ice-cold 0.83 M sucrose buffer (0.83 M sucrose, 20 mM Tris-HCl, 2 mM Na 2 EDTA, pH 7.45) per tube, with care taken to avoid mixing between layers

Glutathione is a potent antioxidant that defends against free radical damage from UV exposure and pollution

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Folic acid can improve hematologic indices in B12 deficiency while allowing neurologic complications to progress, so proper diagnosis and targeted replacement are essential