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Serum and urinary 2 microglobulin levels are elevated in PD patients, particularly those with longer disease duration, suggesting its potential value as a progressionrelated biomarker [150]

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112 More recently, it was suggested that methotrexate causes ferroptosis through activation of autophagy, leading to increased degradation of ferritin and liberation of ferrous iron (ferritinophagy), which promotes LPO and liver injury

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Cells were harvested by centrifugation at 4,400 g at 4 C for 15 min and the medium was discarded