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Acquisition, maintenance and relapse-like alcohol drinking: lessons from the uchb rat line

The role of cystinosin in the intermediary thiol metabolism and redox homeostasis in kidney proximal tubular cells

For this purpose, cells grown on glass coverslips were loaded with fura-2 by a 30 min incubation at 37 C with 1 M fura-2-AM and 0.02% Pluronic in the extracellular solution described above

Since we are unable to digest Glutathione directly, consuming L- Cysteine is one of the ways to do so in order to receive the necessary nutrients

Then, we applied the coupled assay to detect the glyoxalase activity in living cells monitored under the input MG