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Fourth, regarding the microbiota-lipid-depression mediation hypothesis, human intervention data are scarcemost evidence [e.g., (8890) on lipid transformation] comes from germ-free mouse models, but the diversity of the human gut microbiota (e.g., ~1,000 species vs

In brief, 500 cells were seeded per well in 384-well plates, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium

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Lipids 37 , 931934 (2002)