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Equal protein amounts were mixed with 4x NuPage LDS buffer (Thermo Fisher Scientific, NP0007), denatured, and separated by 10% SDS-PAGE

Thus, we determined ER-stress response genes Xbp1 and the ER chaperone heat shock 70 kDa protein 5 (HSPA5, previously known as immunoglobulin heavy chain binding protein [BIP]), inflammation-responsive genes IL6 and IL1, and hypoxia-responsive genes hypoxia-inducible transcription factor 1 (HIF1) and GAPDH

89 Notably, in the landmark study by Cercek et al