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Precursors to Glutathione: Certain supplements, such as N-acetylcysteine (NAC) and alpha-lipoic acid, are glutathione precursors

Recombinant cloning vectors were transformed into Escherichia coli BL21 (DE3) (Stratagene, USA, La Jolla) via electroporation

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Measurement of cell death by PI The experiment was carried out as described previously [37]

While the exact mechanism of melatonin induced apoptosis remains to be elucidated, its pro-oxidant activity, presumably, constitutes a mechanism that may result in tumor cell death by apoptosis