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For breeding to increase FAA content in edamame seeds, the simultaneous introduction of the Shirayama-type qAAD11_1 allele (a loss-of-function allele of TOF11 ) and the Enrei-type qAAD11_2 allele is essential

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Phthalates, which, beyond being used as plasticizers in PVC, polyethylene, polyethylene terephthalate, and polyvinyl acetate to enhance transparency, strength, plasticity, and durability of plastic materials, can also be found in adhesives, cosmetics, fertilizers, food containers, lubricants, medical devices, paints, pesticides, and toys, are characterized by an estimated worldwide production of between 6 and 8 million tons per year [49,50]

Viral cDNA is then incorporated into the host DNA