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Description
Cells were harvested (16,000 x g, 4 C, 2 min) and washed two times with 1 mL washing buffer (50 mM NaCl, 10 mM NH 4 Cl, 10 mM MgCl 2 , 40 mM MOPS, 10 mM IAM, pH 7.3)

Nat Rev Mol Cell Biol 22, 96118, doi: 10.1038/s41580-020-00315-9 (2021)

Cell 81 , 38033819 (2021)
244 mV) similar to the redox potential in the dsbA single mutant (ca

These studies show that people with higher levels of vitamin D have a lower risk of disease, although they do not definitively prove that lack of vitamin D causes disease or that vitamin D supplements would lower risk
