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Western blotting analyses The tissue samples (including human jejunum, mouse intestine tissues, intestinal stem cells and Paneth cells) were lysed in RIPA Lysis Buffer (P0013C, Beyotime) containing 1% PMSF and protease inhibitor cocktail (HY-K0010, MedChemExpress)

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12,1517 Following an overdose, hyperammonemia usually occurs due to the depletion of carnitine stores during the bodys attempt to metabolize an excessive amount of valproic acid

Remarkably, hypoperfusion persisted during acute attacks, with hPBGD mRNA treatment being the sole intervention capable of restoring baseline CBF levels (figure 2E)