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AHK-Cu, or Alanine-Histidine-Lysine-Copper, is a naturally occurring tripeptide complexed with copper that has been investigated for its role in activating copper-dependent enzymatic processes and modulating extracellular matrix proteins involved in tissue structure and repair

Specifically, adding 5-amino-1mq (30 M and above) to C2C12 myoblast cultures (a common in vitro myoblast model) markedly upregulates the expression of myogenic marker genes, including myosin heavy chain (MyHC), actin, and myogenin (MyoG)

Higher NAD+ levels and better mitochondrial function mean cells can respond more robustly to anabolic signals

By preventing NNMT from draining the nicotinamide pool, 5-amino-1mq helps maintain NAD+ at levels that support optimal energy production

[DOI] [PubMed] [Google Scholar] 10.Fildes A, Charlton J, Rudisill C, Littlejohns P, Prevost AT, Gulliford MC