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Metabolic Rearrangement During Differentiation Reflects Changes in the Way Glucose is Utilized Since glucose is the primary energy source for the brain, we performed a flux analysis using 13 C-glucose to examine the incorporation of glucose-derived carbons into downstream metabolites during in vitro neuronal differentiation

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For the construction of the metE mutant, the AES -which contained a Kan R cassette and the GFP gene optimized for its expression in mycobacteria (eGFP) (Supplementary Note 1)- was synthesized by GenScript