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Someone who has dysarthria may experience speech-related symptoms, such as: vocal tremors monotonous vocal tone a voice that sounds hoarse, rough, scratchy, or nasal speech that is unusually quiet or loud speech that is excessively fast or slow twisted vowel and consonant sounds When a person has dysarthria due to Parkinsons disease, they may also experience movement-related symptoms, including: a slowness or difficulty with moving or walking trouble balancing, falling, dizziness, or fainting body stiffness a stooped posture facial masking, which refers to difficulties making facial expressions body tremors It is important to note that Parkinsons disease also may include nonmovement symptoms, such as psychological changes, sleeping issues, loss of smell, constipation, and weight loss

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After blocking, cells were incubated with an anti-BrdU antibody (Thermo Fisher Scientific, MA3-071), followed by incubation with a Fluor 488-conjugated secondary antibody (Abcam, ab150117)

The cell lysates thus obtained were clarified by centrifugation (14,000 g ) in a microcentrifuge at 4 C, and the supernatant was mixed with 25 L of 50% anti-M2 FLAG antibody agarose beads