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After the slides were washed with PBS, the samples were treated with FITC anti-mouse CD86 antibody (1:200, Biolegend, 105006), CD68 antibody (1:200, Biolegend, 130712), CD19 (1:50, eBioscience, 53-0194-82), CD11c (1:50, 43-044 for 30 min, GLUT1 (1:200, Invitrogen, MA5-31960) conjugated with Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 (1:500, Invitrogen, A32732) and Hoechst33342 (1 g/mL) for 5 min prior to the experiment

Setting realistic expectations upfront prevents disappointment and helps people make informed decisions about their treatment approach

Qian H, Chen Y, Nian Z, Su L, Yu H, Chen F-J, et al

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