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Munker R, Zhao S, Jiang S, Snell V, Andreeff M , Andersson BS
doi: 10.3390/molecules24030556 144 Munzurolu.ZenginF
Long-term 3D PureCol EZ Gel (Fujii medium) Ileum (N39) organoids were cultured in expansion medium consisting of DMEM/F12 supplemented with 100 U ml 1 P/S, 10 mM HEPES, 1 Glutamax, 2% B-27 with VitA, 3% (v/v) recombinant R-spondin-3, 2% (v/v) recombinant noggin, 1 mM N -acetylcysteine, 0.5 nM Wnt surrogate, 0.1 g ml 1 hIGF-1 (Peprotech), 0.05 g ml 1 hFGF2 (Peprotech), 10 nM gastrin, 0.5 M A83-01, 0.05 g ml 1 hEGF, 3 M CHIR 99021 and 10 M forskolin (Tocris) 42
Ligand binding to the GIP receptor activates Gas, which in turn activates adenylate cyclase, resulting in the production of cAMP, in accordance with GLP- 1 receptor activation
Moreover, high cell viability, excellent cell adhesion and maturation and easy synchronization with grafted tissue were observed (Qasim et al., 2019)
