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Int J Vet Sci

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For sandwich ELISA, Maxisorp plates were coated overnight at 4 C with a polyclonal goat anti-HSA antibody (Sigma, # A-7544) diluted 1:1000 in PBS for the unfused HSAs or with monoclonal rabbit anti-camelid VHH antibody (Genscript, # A01860) diluted 1:1000 in PBS for the Albu-LiTE fusion proteins

Collectively, these molecular pathways converge to attenuate oxidative damage, suppress apoptotic signaling, and preserve oocyte developmental competence during aging

Beyond disease-specific outcomes, accumulating evidence indicates that luteolin modulates core mechanisms governing folliculogenesis and follicular survival