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The bead mixture was eluted with 75 L of Monarch RNA Cleanup Binding Buffer (0.5 mg/ml N6-Methyladenosine) with gentle rotation at RT for 1 h
International journal of molecular sciences, 23(19), 11991
Then, pre-warmed DMEM supplemented with 2% FBS and 2% carboxymethylcellulose (CMC) was gently added to the plates and incubated for 4 days at 37 C and 5% CO 2 to allow the viral plaque formation
Mood Swings and Irritability Emotional instability, including mood swings and irritability, is frequently reported
