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Assessment of cell differentiation The differentiated C2C12 cells were stained with Crystal Violet Staining Solution (Beyotime, China), and images were taken using an inverted microscope (Nikon ECLIPSE Ti, Japan)

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All methods and procedures were reviewed and approved by the Institutional Review Board of Springfield College and conducted in accordance with the Declaration of Helsinki

The Hs-CRP causes increased mobilization of monocytes into atheromatous plaque, which leads to suppression of basal and induced nitric oxide release from vascular endothelium, which finally causes endothelial dysfunction

This is because creatine is present in large quantities in high protein sources