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Sorafenib (SF) is an oral tyrosine kinase inhibitor that induces GPX4 inactivation by blocking system Xc- and promotes ferroptosis (Zheng et al., 2021a)

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doi:10.1164/rccm.201401-0158OC Semler MW, Bernard GR, Aaron SD, et al

Interestingly, we have found that Immunocal suppresses lipopolysaccharide-induced nitric oxide production in cultured BV2 mouse microglial cells, suggesting that this whey protein may possess some anti-neuroinflammatory properties (Khatter and Linseman, unpublished data)

After various treatments, 10 l of CCK8 solution was added to each well and incubated for 2 hours at 37C