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Lifestyle Adjustments: Limiting time spent in front of bright screens, wearing a hat or sunglasses outdoors, and taking regular breaks from computer work are all effective ways to manage sensitivity to light

After IEF, the IPG gel strips were equilibrated twice for 15 min under gentle shaking at room temperature, first in a solution (equilibration buffer: 50 mM TrisHCl, pH 8.8, 6 M urea, 30% glycerol, 1% w/v SDS containing 1% dithiothreitol (DTT), then in an equilibration buffer containing 2.5% iodoacetamide

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doi:10.1002/14651858.CD004326

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