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The same solution (250 mM mannitol, 5 mM HEPES (pH 7.4) and 0.5 mM EGTA) was gently layered on the top to fill up the centrifuge tube and was centrifuged at 95,000 g for 30 minutes (Beckman Coulter Optima L-100 XP Ultracentrifuge (SW40 rotor, Beckman, Fullerton, CA, USA)
The culture medium was used within 2 weeks to prevent L-glutamine degradation

An Oral Combination Study of Novel Nucleoside Analogue Sapacitabine and BCL2 Inhibitor Venetoclax to Treat Patients with Relapsed or Refractory AML or MDS

IV treatment involves no downtime

doi: 10.1021/acscentsci.0c01306
