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Ferrous ions (Fe 2 ) are potent lipid oxidation pro-oxidants, stimulating ROS production and damaging lipids, proteins, and nucleic acids (Halliwell and Gutteridge 1984)

Safety and efficacy of omaveloxolone in Friedreich ataxia (MOXIe Study)

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Astragaloside IV can also enhance pancreatic beta cell dysfunction induced by uric acid through the activation of the PI3K/AKT pathway, anti-apoptotic effects, and activation of autophagy (Zhang et al., 2021)

Coimmunoprecipitation and Western blotting For coimmunoprecipitation assays, cells were washed twice with PBS and lysed with an NP-40 buffer (100 mM NaCl, 10 mM TrisHCl, pH 7.5, 0.2% NP-40, 10% glycerol) supplemented with cOmplete protease inhibitor cocktail (MilliporeSigma, Roche)