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Polyhistidine tags containing less than six histidines typically require less imidazole for elution, while polyhistidine proteins containing more than six polyhistidines may require higher levels of imidazole

GPx3 has been reported to be a glycoprotein 12 , but rhGPx3 mutant produced in E.coli lacked such post-translational glycosylation because of the difference between prokaryotic and eukaryotic cells

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Gwyer, Wragg & Wilson (2019), "Gastric pentadecapeptide body protection compound BPC 157 and its role in accelerating musculoskeletal soft tissue healing," Cell and Tissue Research 377(2):153-159 [PubMed]

This is the concept of bioavailability , which refers to the proportion of a nutrient that enters the circulation and is able to have an active effect