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The supernatant was replaced by 35 mL of isotonic resealing medium (NaCl 145 mM, HEPES 5 mM, glucose 5 mM, pH 7.4, 300 mOsm/kg H2O , 37C)

Adding to the paracellular movement of drugs across capillaries is the bulk flow of water that moves in relation to the net balance of hydrostatic and osmotic forces between the vascular and interstitial compartments