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Fetal reversion enables villus-derived cells to regenerate tissue without exogenous oncogenic mutations To determine whether the fetal reversion and subsequent conversion to CBCs observed in Alpi+ enterocytes in vitro could also occur under more physiological conditions, we performed primary collagen culture of villus tissues containing tdTomato-labeled Alpi+ cells, followed by in vitro organoid expansion and subsequent transplantation experiments using a DSS-induced colitis model 23,24

For zebrafish cell line and bioluminescence assays, the generation of a per1b-luciferase cell line from zebrafish PAC2 cells has been previously described 62

In an evaluation of protein oxidation detected using a fluorescent probe targeted to sulfenic acid (DCP-Rho1) applied to cells activated with the lipid growth signal lysophosphatidic acid (LPA), our group recently found evidence for enhanced protein oxidation around redoxosomes (ROS-generating, signaling endosomes) which were also enriched in internalized LPA receptors [134]

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However, complete blockage of ECM attachment can be expected upon filling of type 2, 3 and 5 sites, as they contain Asp256 and Asp258, or Asp 300, which are directly involved in collagen binding 86