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Functional annotation highlighted genes potentially involved in AFB1 detoxification, including predicted cytochrome P450, epoxide hydrolase (LAP2), glutathione-S-transferase (URE2), and the ABC transporter (YCF1), suggesting pathways for AFB1 activation, glutathione conjugation, and vacuolar sequestration

The study also explores the AITC and protein-protein interaction among pepsin and mustard seed proteins, cruciferin and napin, as mustard contains abundant protein content but remains bio-unavailable due to presence of similar anti-nutritional factors like glucosinolates potentially having insect deterring potential

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