does ghk-cu stimulate pituitary gh release Therapeutic Peptides in Aesthetic, Metabolic and Endocrine Conditions: Effects, Safety, Clinical Applications, and Future Perspectives Ghrelin acts within the hypothalamus
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Immunostaining was performed by seeding cells on glass coverslips, fixing with paraformaldehyde, permeabilizing with Triton X-100, and then incubating with primary anti-NF-B p65 antibody, followed by Alexa Fluor 546-conjugated secondary antibody

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Cell 104 , 165172 (2001)
