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BGI (Yantian District, Shenzhen, China), an established contract research organization, isolated DNA from the remaining cecal samples and generated a metagenomic 16s rDNA amplicon library

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As confirmation, we used the crisis that occurred with the high intra-abdominal pressure-induced syndrome, in which intra-abdominal hypertension simultaneously affected all abdominal vessels and organs for a considerable period and restrained the ability to recruit alternative pathways, such that a deadly situation was created before therapy initiation

For hematocrit and reticulocyte counts, recommendations are to repeat these tests daily from the 5th to 7th days of treatment and then frequently until the hematocrit returns to a normal range